Measurement

Part:BBa_K3187011:Design

Designed by: iGEM TU_Darmstadt 2019   Group: iGEM19_TU_Darmstadt   (2019-10-12)


pT7 Superfolder Green Fluorescence Protein x pTet mCherry (Convergent)


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 76
    Illegal XbaI site found at 1729
    Illegal PstI site found at 805
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 76
    Illegal NheI site found at 810
    Illegal PstI site found at 805
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 76
    Illegal XhoI site found at 970
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 76
    Illegal XbaI site found at 1729
    Illegal PstI site found at 805
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 76
    Illegal XbaI site found at 1729
    Illegal PstI site found at 805
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 1674


Design Notes

Codon-optimized for E. coli


Source

Ordered from Integrated DNA Technologies (IDT)

References